Steps!
1. On the Entrez Gene website search the term “human actin”.
2. Click on the fi rst link that appears and scroll down to “NCBI Reference Sequences”.
3. Click on the fi rst fi le name “NM 001100.3” under “mRNA and Proteins”.
4. Click on “FASTA” just below the gene title.
5. The nucleotide sequence displayed is that of the actin gene in humans.
6. Copy the gene sequence and go to the BLAST homepage (http://blast.ncbi.nlm.nih.
gov/Blast.cgi).
7. Click on “nucleotide blast” under the Basic BLAST menu.
8. Paste the sequence into the box where it says “Enter Query Sequence.”
9. Give the query a title in the box provided if you plan on saving it for later.
10. Under “Choose Search Set” select the type or genome you want to search (human
genome, mouse genome, or all genomes available).
11. Under “Program Selection” choose whether or not you want highly similar sequences
or somewhat similar sequences. Choosing somewhat similar sequences will provide
you with more results.
12. Click BLAST.
2. Click on the fi rst link that appears and scroll down to “NCBI Reference Sequences”.
3. Click on the fi rst fi le name “NM 001100.3” under “mRNA and Proteins”.
4. Click on “FASTA” just below the gene title.
5. The nucleotide sequence displayed is that of the actin gene in humans.
6. Copy the gene sequence and go to the BLAST homepage (http://blast.ncbi.nlm.nih.
gov/Blast.cgi).
7. Click on “nucleotide blast” under the Basic BLAST menu.
8. Paste the sequence into the box where it says “Enter Query Sequence.”
9. Give the query a title in the box provided if you plan on saving it for later.
10. Under “Choose Search Set” select the type or genome you want to search (human
genome, mouse genome, or all genomes available).
11. Under “Program Selection” choose whether or not you want highly similar sequences
or somewhat similar sequences. Choosing somewhat similar sequences will provide
you with more results.
12. Click BLAST.
Materials:
- blastlab website
- chromebook/notes for info
- google or any search engine of your preference
- gene of your choice
Question:
In humans, what is the importance of the gene you chose?
I belive that the importace of the gene chosen is if it is a strong characteristic. It is an enzyme that catalyzes the reduction of hydrogen peroxide. Our body produces toxic substances every day. One reason they don't harm us is because of catalase. Catalase is an enzyme that destroys harmful substances inside your body.
Would you expect to find that gene is all organisms? Why or why not?
I think mostly all organisms wil carry the trait because if it was only common in some organisms then there will be a lot of mutations in DNA.
Hypothesis:
The gene I chose is Catalase. If the gene catalase is an extremely beneficial gene then it should be found in all organisms and the only way it will not apear is due to mutations.
,
In humans, what is the importance of the gene you chose?
I belive that the importace of the gene chosen is if it is a strong characteristic. It is an enzyme that catalyzes the reduction of hydrogen peroxide. Our body produces toxic substances every day. One reason they don't harm us is because of catalase. Catalase is an enzyme that destroys harmful substances inside your body.
Would you expect to find that gene is all organisms? Why or why not?
I think mostly all organisms wil carry the trait because if it was only common in some organisms then there will be a lot of mutations in DNA.
Hypothesis:
The gene I chose is Catalase. If the gene catalase is an extremely beneficial gene then it should be found in all organisms and the only way it will not apear is due to mutations.
,
Justification: | What I found about my gene Catalase, in the cladogram is that it is found commonly in plants. The plants that I found Catalase is mostly in "eudicots" and in "arabidopsis." |